Flow cytometry was born out of impedance technology; in 1953, a patent was issued to American
for a cell counter based on the 'Coulter Principle'. This device was the forerunner to modern impedance cell analysers, flow cytometry and cell-sorting devices.
Today, flow cytometry is one of the most ubiquitous tools in bioscience. It has applications in molecular biology, immunology, plant and marine biology, medicine (particularly haematology and pathology), protein engineering and cell biology research, among others.
we have over a decade of experience in impedance, microfluidics and advanced electronics research and development. Our recent R&D advances led to the development of the
- bringing impedance-based flow cytometry to the cutting edge of cell-based analysis.
can analyse and sort cells based on various factors, such as size, morphology, cell cytoplasm conductivity, organelle impedance, cellular DNA content, and cell membrane integrity.
can also differentiate membranes based on health - intact membranes, damaged membranes, dead cells and, in a first-in-field innovation, is capable of differentiating cells with open membrane.
Biological membranes have varied roles, and understanding them is integral to our understanding of the cell.
The gradient of ions across membranes allows for energy generation, the most essential of all life processes. In addition, membranes control cell-cell communication via chemical and electrical signals, and allow separation between from the environment. Membrane health is required for normal cellular homeostasis, and membrane repair is essential is for cell survival - in fact, defective membrane repair can contribute to the pathology of diseases such as muscular dystrophy.
can separate populations into live-dead-open membrane-damaged membrane subsets. This allows quantification of cell health in unprecedented detail, and separation based on this - all in a label-free manner so as to preserve cell normal function.
This technology has profound implications for basic and applied cell biology research. For example, this open-membrane quantification is ideal for transfection experiments - understand precisely the effect of any electroporation processes on the cell membrane, and streamline your experiment optimisation!
is an impedance-based cell analysis and cell counting tool with a range of applications... Even better, we are willing to customise the technology for your use; for an applied example of the
From quantifying cells suitable for transfection to counting nanoplankton; our latest developments in label-free impedance flow-cytometry may be of use to you.
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Innovations in Flow Cytometry: The Inish Analyser
Vivienne Williams
May 3, 2018
2 min read
Updated:
Aug 28, 2020
History and Applications of Flow Cytometry
Flow cytometry was born out of impedance technology; in 1953, a patent was issued to American
Wallace H. Coulter
for a cell counter based on the 'Coulter Principle'. This device was the forerunner to modern impedance cell analysers, flow cytometry and cell-sorting devices.
Today, flow cytometry is one of the most ubiquitous tools in bioscience. It has applications in molecular biology, immunology, plant and marine biology, medicine (particularly haematology and pathology), protein engineering and cell biology research, among others.
Here at
Cellix,
we have over a decade of experience in impedance, microfluidics and advanced electronics research and development. Our recent R&D advances led to the development of the
Inish Analyser
- bringing impedance-based flow cytometry to the cutting edge of cell-based analysis.
Cell Sorting
The
Inish Analyser
can analyse and sort cells based on various factors, such as size, morphology, cell cytoplasm conductivity, organelle impedance, cellular DNA content, and cell membrane integrity.
The
Inish Analyser
can also differentiate membranes based on health - intact membranes, damaged membranes, dead cells and, in a first-in-field innovation, is capable of differentiating cells with open membrane.
Quantification of Membrane Health
Biological membranes have varied roles, and understanding them is integral to our understanding of the cell.
The gradient of ions across membranes allows for energy generation, the most essential of all life processes. In addition, membranes control cell-cell communication via chemical and electrical signals, and allow separation between from the environment. Membrane health is required for normal cellular homeostasis, and membrane repair is essential is for cell survival - in fact, defective membrane repair can contribute to the pathology of diseases such as muscular dystrophy.
Uniquely, the
Inish Analyser
can separate populations into live-dead-open membrane-damaged membrane subsets. This allows quantification of cell health in unprecedented detail, and separation based on this - all in a label-free manner so as to preserve cell normal function.
This technology has profound implications for basic and applied cell biology research. For example, this open-membrane quantification is ideal for transfection experiments - understand precisely the effect of any electroporation processes on the cell membrane, and streamline your experiment optimisation!
Applications of the Inish Analyser
The
Inish Analyser
is an impedance-based cell analysis and cell counting tool with a range of applications... Even better, we are willing to customise the technology for your use; for an applied example of the
Inish Analyser technology
, see our innovative
Inish MiniBar.
From quantifying cells suitable for transfection to counting nanoplankton; our latest developments in label-free impedance flow-cytometry may be of use to you.
Curious?
Contact us about a free in-house demo of our Inish Analyser.
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#CellSorting
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Gene Transfection
Label Free Analysis
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