Cell counts and cell viabilities of cell lines are key metrics of every cell-based assay and analysis of these metrics are performed daily in thousands of labs around the world. Cell lines are particularly important for R&D applications including testing
Transfection efficiencies of cells are particularly important in research applications and poor transfection efficiencies can prove to be very time-consuming. The Inish Analyser
) assay improves workflows ensuring you are plating the highest number of transfected cells.
The Inish Analyser does not require any expensive consumables such as slides or cartridges. Instead we use affordable standard tubes (500 µL), available from Cellix. Sample preparation is very simple - just using a pipette.
Cell Sample Preparation (Cell Counting & Viability and Transfection Efficiency Assays)
In this example, we use Jurkat cells. Culture the Jurkat cells in the recommended medium RPMI 1640 (Gibco 31870) supplemented with L-glutamine (Sigma 59202C) and 10% Fetal Bovine Serum (Sigma F4135). Centrifuge the Jurkat cells at 50g for 8 minutes. After centrifugation, resuspend the cells in growth media to ensure the cells are in suspension. Recommended cell concentration is 50,000 - 2,000,000 cells/mL. Vortex cells or pipette cells up and down to ensure a homogenous solution.
L of sample and mix gently. Transfer cell/DNA suspension into certified cuvette and select the appropriate Nucleofector Programs for high viability (X-01) and high expression (X-05). Follow the steps below to execute the
L pre-warmed antibiotic free media to each of the remaining transfected samples. Wait 10 minutes before plating.
The protocol for running an assay with the Inish Analyser is the same for both the Cell Counting & Viability assay and the Transfection Efficiency Prediction assay.
Results of Cell Counting & Viability and Transfection Efficiency Assays
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CELL LINES
Cell counts and cell viabilities of cell lines are key metrics of every cell-based assay and analysis of these metrics are performed daily in thousands of labs around the world. Cell lines are particularly important for R&D applications including testing
drug metabolism and cytotoxicity.
Transfection efficiencies of cells are particularly important in research applications and poor transfection efficiencies can prove to be very time-consuming. The Inish Analyser
Transfection Efficiency Prediction
(
TEP
) assay improves workflows ensuring you are plating the highest number of transfected cells.
Get a Quote or more information
What do I need to get started?
Downloads
Related Blogs
Recommended Products
WHAT DO I NEED TO GET STARTED?
Materials
The Inish Analyser does not require any expensive consumables such as slides or cartridges. Instead we use affordable standard tubes (500 µL), available from Cellix. Sample preparation is very simple - just using a pipette.
In general, you will need the following to execute
Cell Counting & Viability
and
Transfection Efficiency
assays:
Cell Sample Preparation (Cell Counting & Viability and Transfection Efficiency Assays)
In this example, we use Jurkat cells. Culture the Jurkat cells in the recommended medium RPMI 1640 (Gibco 31870) supplemented with L-glutamine (Sigma 59202C) and 10% Fetal Bovine Serum (Sigma F4135). Centrifuge the Jurkat cells at 50g for 8 minutes. After centrifugation, resuspend the cells in growth media to ensure the cells are in suspension. Recommended cell concentration is 50,000 - 2,000,000 cells/mL. Vortex cells or pipette cells up and down to ensure a homogenous solution.
Transfection Step - Electroporation (Transfection Efficiency Assay)
Add 2
µ
g (4
µ
L) of DNA (GFP vector) to each 100
µ
L of sample and mix gently. Transfer cell/DNA suspension into certified cuvette and select the appropriate Nucleofector Programs for high viability (X-01) and high expression (X-05). Follow the steps below to execute the
Transfection Efficiency Prediction (TEP)
assay with the Inish Analyser. Should satisfactory
TEP
be detected, add 100
µ
L pre-warmed antibiotic free media to each of the remaining transfected samples. Wait 10 minutes before plating.
Running an Assay with the Inish Analyser
The protocol for running an assay with the Inish Analyser is the same for both the Cell Counting & Viability assay and the Transfection Efficiency Prediction assay.
Results of Cell Counting & Viability and Transfection Efficiency Assays
Cell Counting & Viability of Jurkat T-cells
Transfection Efficiency of Jurkat T-cells (high viability)
Transfection Efficiency of Jurkat T-cells (high expression)
Cell Counting & Viability of Jurkat T-cells
What do I need to get started?
DOWNLOADS
Downloads
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