DropChipA world inside a droplet

DropChip

3 Droplet generators and flow splitter on one chip

DROPLET GENERATION ASSAYS

with oil and water phases producing uniform, stable,

mono-dispersed

droplets.

DROP-SEQ (CELL & PARTICLE ENCAPSULATION) ASSAYS

easily achieved with splitter and dual-injection flow focusing droplet generator.

REUSABLE & COST-EFFECTIVE:

1 x Flow splitter and 3 x Droplet Generator junctions on-chip with 2 different designs for single or dual injection of dispersed phase.

FLOW SPLITTER & FLOW FOCUSING JUNCTIONS

on one single chip enabling a wide range of experimental set-ups.

PLUG & PLAY

with easy-to-connect tubing with standard 25G needles, no bulky luer connectors.

COMPATIBLE WITH MICROFLUIDIC SYRINGE PUMPS & MICROFLUIDIC PRESSURE PUMPS

including Cellix's ExiGo, UniGo and 4U pumps for precise pulse-free stable flow rates.

COMPATIBLE WITH STANDARD PIPETTES

for easy coating of chips with reagents, e.g. to ensure channel hydrophobicity for water-in-oil droplet generation.

COMPATIBLE WITH IMMUNOFLUORESCENCE MICROSCOPY

for cell encapsulation studies.

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How does it work?

Applications

Related Products & Accessories

Demo Videos

Training Videos

HOW DOES IT WORK?

DropChips

are fabricated from plastic (acrylic) and have a footprint of 40mm x 50mm (W x L).

DropChips are available with channels pre-treated as hydrophobic or hydrophilic:

Hydrophobic

to achieve

water-in-oil droplets

.

Hydrophilic

to achieve

oil-in-water droplets

.

DropChips are available in packs of 2, 5 or 10.

Hydrophobic channels:

Water-in-oil droplet generation

Hydrophilic channels:

Oil-in-water droplet generation

As illustrated in the schematic below, each DropChip contains:

1 x Flow Splitter

and

3 x Droplet Generator junctions:

2 x Flow-Focusing junctions, single-injection dispersed phase

1 x Flow Focusing junction with dual-injection dispersed phase

This gives the user flexibility for experiments in flow focusing with single and dual-injection of dispersed phase.

Examples are illustrated in the images below.

DropChip Schematic

3 Flow Focusing Droplet Generator junctions & 1 Flow Splitter

DropChip Flow focusing junction

Single injection of water-in-oil droplets containing cells

DropChip Flow focusing junction

Dual injection of water-in-oil droplets containing cells with reagent

DropChip Schematic

3 Flow Focusing Droplet Generator junctions & 1 Flow Splitter

Droplet Frequency

Droplet Size

DOWNLOADS

DropChip Tech Note

Droplet Generation Kit Brochure

Droplet Frequency

The creation of uniform, mono-dispersed  stable droplets is achieved with microfluidic syringe pumps such as Cellix's ExiGo pump or microfluidic pressure pumps such as Cellix's UniGo or 4U pumps.  Cellix's team conducted 3D scattering measurements to confirm frequency of droplets which can be achieved at defined flow rates as illustrated in the images and frequency graph below.  If you have a specific frequency you want to achieve,

contact us

for more help.

Droplet Frequency in DropChip  with channel

dimensions

70um x 70um

Oil phase: 5uL/min

Water phase: 0.5uL/min

Oil phase: 5uL/min

Water phase: 1uL/min

Oil phase: 5uL/min

Water phase: 2uL/min

Oil phase: 5uL/min

Water phase: 3uL/min

Droplet Size

There are a variety of different droplet sizes possible and one of the biggest influencing factors of droplet size is the geometry and size of the channel dimensions.  Cellix's team conducted 3D scattering measurements to confirm size and volume of droplets which can be achieved with the two different types of droplet generator contained in the DropChip.  Schematics for each of the droplet generators available on Cellix's Dropchip are illustrated below and the following table provides a summary of the droplet sizes (diameter and volume) which may be achieved depending on the DropChip dimensions.

Droplet Generator junction 1 & 3 (DG1 and DG3):

Flow Focusing, single injection of dispersed phase

Droplet Generator junction 2 (DG2):

Flow Focusing, dual injection of dispersed phase

How does it work?

Droplet Frequency

Droplet Size

APPLICATIONS

Droplet Generation

for a wide variety of applications:

Drop-Seq (Cell / Protein) encapsulation on-chip, see example below.

DNA sequencing for target selection / enrichment.

Sample / reagent mixing and control of reactions on-chip

DNA amplification

Biomolecule synthesis

Diagnostic chips

Drug discovery

For more details on experimental set-ups, visit our

Droplet Generation

page.

APPLICATION VIDEOS

DropChip - Droplet Generation with ExiGo pump

Droplet Generation with ExiGo Microfluidic Pumps

DropChip - Droplet Generation with ExiGo pump

Cell (or particle) double encapsulation

:

The image below illustrates droplets containing cells, enclosed within a shell of oil.  The following is an example of the experimental set-up to achieve double encapsulation.

Experimental Set-up

3 x ExiGo pumps

3 x Flow Sensors

1 x DropChip (50um x 50um) coated with DropCoat (reagent ensuring hydrophobicity)

1 x DropChip (80um x 80um) uncoated, hydrophilic walls

Applications

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